Both boards share the same AM5 socket and B850 chipset, meaning they support the same generation of AMD processors and offer identical overclocking headroom. They also match on HDMI 2.1, dual BIOS, easy BIOS reset, RGB lighting, and a 3-year warranty — so neither board has a platform-level or support advantage over the other. The most immediately practical difference is form factor: the Asus Prime B850-Plus Wi-Fi is a full ATX board (305 × 244 mm), while the TUF Gaming B850M-Plus Wi-Fi 7 is Micro-ATX (244 × 244 mm). This affects case compatibility and, to a lesser extent, the number of expansion slots available — ATX typically offers more PCIe real estate, while Micro-ATX suits smaller, more compact builds.
The more meaningful differentiator in this group is wireless connectivity. The TUF Gaming supports Wi-Fi 7 (802.11be), whereas the Prime tops out at Wi-Fi 6E. In practical terms, Wi-Fi 7 delivers significantly higher theoretical throughput, lower latency, and introduces multi-link operation — benefits that matter most if you have a Wi-Fi 7 router and a bandwidth-intensive use case. The TUF Gaming also edges ahead on Bluetooth, offering version 5.4 versus 5.3 on the Prime; the difference is minor in day-to-day use, but 5.4 brings marginally improved connection stability and energy efficiency.
For this spec group, the TUF Gaming B850M-Plus Wi-Fi 7 holds a clear advantage on wireless: Wi-Fi 7 and Bluetooth 5.4 are objectively newer standards. However, if your build requires a full-ATX board for case compatibility or expansion slot density, the Prime's larger form factor is the deciding factor regardless of wireless. Choose the TUF Gaming if wireless performance and compact sizing are priorities; choose the Prime if you need ATX and can live without Wi-Fi 7.